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PhD position BAT IIa-O (60%) (Invalidenstr. 42, D-10115 Berlin) PDF Print E-mail
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Saturday, 24 April 2010 20:46

Position BAT IIa-O (60%) available for three years (DFG funded)
Project Title
Interaction of ECF and ABC transporter modules studied by time resolved fluorescence microscopy and spectroscopy

Summary
The organisation and structure of an bacterial ABC transporter during the catalytic cycle of the transporters will be investigated by time resolved fluorescence microscopy and spectroscopy. Various techniques including Fluorescence Lifetime Imaging, Förster-Resonance-Energy-Transfer, Fluorescence (Cross) Correlation spectroscopy and Fluorescence Recovery after Photobleaching will be employed to study the interaction of the various subunits and modules of transporters and their conformational dynamics. Transporter subunits will be labelled either by fluorescent proteins or by reactive fluorophores. By tagging of transporter subunits with fluorescent proteins, measurements can be performed on living cells (E. coli).

Qualifications:
PhD preferable in Biophysics (or Biochemistry, Biology), experiences/ knowledge in optical spectroscopy, microscopy, protein expression and/or membrane- and vesicle preparation.




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Von:

Prof. Andreas Herrmann
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Humboldt University Berlin, Dept Biology/Molecular Biophysics
Invalidenstr. 42, D-10115 Berlin

Ansprechpartner: Prof. Andreas Herrmann, This e-mail address is being protected from spambots. You need JavaScript enabled to view it

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